Project Overview
N2 C. elegans were randomly mutated, and those that displayed a defective zap response were isolated. The genomes of five of the defective mutant strains were sent to be sequenced. In order to find the causative mutation in each, students are using Galaxy to determine the sequencing differences in comparison to the original N2 genome.
Why?
An important aspect of “why” relates to one of the five mutants sequenced: J102.
In J102, it was discovered that sma-1 is the causative gene, thus the Zap and Food & Zap Response teams are able to focus on strains with applicable traits (size, cuticle, excretory canals) to determine the character responsible for the defective zap response.
Identifying the causative genes in LF61, LF71, J71, and J72 will enable further studies to support the complementation analysis previously done as well.
The Mapping-By-Sequencing Team!
Julia Carter, Jory Gutman, Gabrielle H. Martin, & Charlotte Roth